TABLE 2.
TnTIR transposition sites and sequences in target plasmids pBAD18-Kan and pDM4
| Plasmid | Region | Sequence (5′-3′) |
|---|---|---|
| pBAD18-Tn1 | araC | GAATA |
| pBAD18-Tn2 | araC | TATTT |
| pBAD18-Tn3 | araC | AAATA |
| pBAD18-Tn4 | rrnB | GAGTA |
| pBAD18-Tn5 | M13 origin | TTATA |
| pBAD18-Tn6 | M13 origin | TTAAT |
| pBAD18-Tn7 | M13 origin | GATTT |
| pBAD18-Tn8 | M13 origin | AGCTT |
| pBAD18-Tn9 | pBR322 origin | ATCTA |
| pBAD18-Tn10 | pBR322 origin | AAGCT |
| pBAD18-Tn11 | PBAD promoter | GGTAT |
| pBAD18-Tn12 | Kanr | TTTTA |
| pBAD18-Tn13 | Kanr | TGATA |
| pBAD18-Tn14 | Kanr | AACAC |
| pBAD18-Tn15 | Kanr | ATGAG |
| pBAD18-Tn16 | Kanr | TATTA |
| pBAD18-Tn17(L) | Multiple cloning site | CTGAT |
| pBAD18-Tn17(R) | rrnB | AAGCT |
| pDM4-Tn1 | RP4 mob region | TATAT |
| pDM4-Tn2 | RP4 mob region | GACCA |
| pDM4-Tn3 | RP4 mob region | AAGCA |
| pDM4-Tn4 | RP4 mob region | TATAA |
| pDM4-Tn5 | RP4 mob region | AAATA |
| pDM4-Tn6 | RP4 mob region | TTATT |
| pDM4-Tn7 | RP4 mob region | TGTAT |
| pDM4-Tn8 | Inserted sequence | TGCAA |