FIG. 1.

Construction of the ΔehbF:pac mutation. A. M. maripaludis S2 ehb operon (subunits E to O; Mmp1629 to Mmp1621). The ORFs Mmp1630, Mmp1626, and Mmp1620 were annotated as ATP/GTP-binding site motif A (P-loop):ABC transporter:AAA ATPase, conserved hypothetical protein, and hypothetical protein (9), respectively. The location of the primers U1, U2, D1, and D2 used to clone the upstream and downstream regions flanking ehbF are shown. The homologous portion of the DNA from M. maripaludis JJ cloned in pIJA10 is shown. B. Confirmation of the genotypes of the wild-type S2 and mutant S40 by Southern hybridization. The genomic DNA (1 μg) was digested with BglII and EcoRV prior to hybridization with the probe indicated in panel A. Lanes 1 and 2, digested genomic DNA of S2 and S40.