FIG. 6.
ParC requires FtsK to localize to the replisome. Mixed cultures of LS4209 (A) and LS4208 (B) were grown in minimal medium at the permissive temperature (28°C) (0 h) to log phase and then shifted to the nonpermissive temperature (37°C) for 4 h. Cells were incubated with 0.3% xylose for 2 h before each time point to induce expression of ftsK-gfp. Top, DIC images; middle, GFP fluorescence; bottom, schematic diagrams of FtsK-GFP fluorescence in black. White scale bar, 2 μm. (C) A mixed culture of LS4207 was grown in rich medium with 0.3% xylose (PYEX) to log phase and then depleted for the FtsK C terminus by growth in rich medium with 0.2% glucose (PYEG) for the indicated number of hours. Before each time point, cells were also incubated with 0.5 mM Na-vanillate (pH 7.5) for 1 h to induce expression of parC-gfp. Top, DIC images; middle, GFP fluorescence; bottom, schematic diagrams of ParC-GFP fluorescence in black. The percentage of cells with ParC-GFP foci in strain LS4207 after depletion of the FtsK C terminus for the indicated number of hours is indicated below the images (n > 140 cells). ts, temperature sensitive.