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. 2006 Feb;188(4):1497–1508. doi: 10.1128/JB.188.4.1497-1508.2006

FIG. 6.

FIG. 6.

ParC requires FtsK to localize to the replisome. Mixed cultures of LS4209 (A) and LS4208 (B) were grown in minimal medium at the permissive temperature (28°C) (0 h) to log phase and then shifted to the nonpermissive temperature (37°C) for 4 h. Cells were incubated with 0.3% xylose for 2 h before each time point to induce expression of ftsK-gfp. Top, DIC images; middle, GFP fluorescence; bottom, schematic diagrams of FtsK-GFP fluorescence in black. White scale bar, 2 μm. (C) A mixed culture of LS4207 was grown in rich medium with 0.3% xylose (PYEX) to log phase and then depleted for the FtsK C terminus by growth in rich medium with 0.2% glucose (PYEG) for the indicated number of hours. Before each time point, cells were also incubated with 0.5 mM Na-vanillate (pH 7.5) for 1 h to induce expression of parC-gfp. Top, DIC images; middle, GFP fluorescence; bottom, schematic diagrams of ParC-GFP fluorescence in black. The percentage of cells with ParC-GFP foci in strain LS4207 after depletion of the FtsK C terminus for the indicated number of hours is indicated below the images (n > 140 cells). ts, temperature sensitive.

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