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Identification of DES-generated mutations by complementation. A) Bracketed region suggests the presence of a 44-kb recombination fragment. A donor chromosome with a mutation in a MEP pathway gene, different (isp2) than the unidentified mutants (isp1), produces a recombination fragment able to repair the unidentified mutation. B) A donor chromosome with a mutation in the same gene as the recipient chromosome does not produce a recombination fragment able to repair the lethal mutation.
