TABLE 1.
Plasmids
Plasmid | Relevant characteristics | Source or reference(s) |
---|---|---|
pDAG203 | Mini-F Δ(sopOPABC) cat+ (6.67 kb) | 30 |
pDAG123 | pRS551 (reppMB1bla+ KanrlacZYA) carrying pldc (12.6 kb) | 31, 47 |
pAM238 | reppSC101aadA; polylinker of pUC18 (4.3 kb) | 8 |
pBBR1MCS5 | reppBBR1mobRK2 Genr (4.77 kb) | 24 |
pDAG551 | pDAG203 with single c1 parS between AflIII-SexAI | This work |
pDAG552 | pDAG203 with single c2 parS between AflIII-Bsu36I | This work |
pDAG553 | pDAG203 with single c3 parS between AflIII-Bsu36I | This work |
pDAG554 | pDAG203 with single p1 parS between AflIII-Bsu36I | This work |
pDAG555 | pDAG203 with c2 parS cluster between PmlI-Bsu36I | This work |
pDAG556 | pDAG203 with c3 parS cluster between PmlI-Bsu36I | This work |
pDAG557 | pDAG203 with p1 parS cluster between PmlI-Bsu36I | This work |
pDAG558 | pAM238 with c1 parAB fragment (including 91 bp upstream of parA) between XbaI-SphI | This work |
pDAG559 | pAM238 with c2 parAB fragment (including 81 bp upstream of parA) between XbaI-HindIII | This work |
pDAG560 | pAM238 with c3 parAB fragment (including 141 bp upstream of parA) between XbaI-SphI | This work |
pDAG561 | pAM238 with p1 parAB fragment (including 126 bp upstream of parA) between XbaI-SphI | This work |
pDAG562 | pBBR1MCS5 with c1 parAB fragment (including 256 bp upstream of parA) between SpeI-HindIII | This work |
pDAG563 | pBBR1MCS5 with c2 parAB fragment (including 81 bp upstream of parA) between XbaI-BamHI | This work |
pDAG564 | pBBR1MCS5 with c3 parAB fragment (including 267 bp upstream of parA) between XbaI-BamHI | This work |
pDAG565 | pBBR1MCS5 with p1 parAB fragment (including 126 bp upstream of parA) between XbaI-BamHI | This work |
pDAG566 | pDAG563 with G→A in central CG of internal parS* | This work |
pDAG567 | pDAG565 with C→T in central CG of internal parS | This work |
pDAG568 | pDAG564 with T→A mutation of promoter −10 (TAAAATa) in c3 parA | This work |
pDAG571 | pDAG123 with single c1 parS at EcoRI upstream of pldc::lacZYA | This work |
pDAG572 | pDAG123 with single c2 parS at EcoRI upstream of pldc::lacZYA | This work |
pDAG573 | pDAG123 with single c3 parS at EcoRI upstream of pldc::lacZYA | This work |
pDAG574 | pDAG123 with single p1 parS at EcoRI upstream of pldc::lacZYA | This work |
The mutated base is underlined.