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. 2006 Feb;188(4):1489–1496. doi: 10.1128/JB.188.4.1489-1496.2006

TABLE 1.

Plasmids

Plasmid Relevant characteristics Source or reference(s)
pDAG203 Mini-F Δ(sopOPABC) cat+ (6.67 kb) 30
pDAG123 pRS551 (reppMB1bla+ KanrlacZYA) carrying pldc (12.6 kb) 31, 47
pAM238 reppSC101aadA; polylinker of pUC18 (4.3 kb) 8
pBBR1MCS5 reppBBR1mobRK2 Genr (4.77 kb) 24
pDAG551 pDAG203 with single c1 parS between AflIII-SexAI This work
pDAG552 pDAG203 with single c2 parS between AflIII-Bsu36I This work
pDAG553 pDAG203 with single c3 parS between AflIII-Bsu36I This work
pDAG554 pDAG203 with single p1 parS between AflIII-Bsu36I This work
pDAG555 pDAG203 with c2 parS cluster between PmlI-Bsu36I This work
pDAG556 pDAG203 with c3 parS cluster between PmlI-Bsu36I This work
pDAG557 pDAG203 with p1 parS cluster between PmlI-Bsu36I This work
pDAG558 pAM238 with c1 parAB fragment (including 91 bp upstream of parA) between XbaI-SphI This work
pDAG559 pAM238 with c2 parAB fragment (including 81 bp upstream of parA) between XbaI-HindIII This work
pDAG560 pAM238 with c3 parAB fragment (including 141 bp upstream of parA) between XbaI-SphI This work
pDAG561 pAM238 with p1 parAB fragment (including 126 bp upstream of parA) between XbaI-SphI This work
pDAG562 pBBR1MCS5 with c1 parAB fragment (including 256 bp upstream of parA) between SpeI-HindIII This work
pDAG563 pBBR1MCS5 with c2 parAB fragment (including 81 bp upstream of parA) between XbaI-BamHI This work
pDAG564 pBBR1MCS5 with c3 parAB fragment (including 267 bp upstream of parA) between XbaI-BamHI This work
pDAG565 pBBR1MCS5 with p1 parAB fragment (including 126 bp upstream of parA) between XbaI-BamHI This work
pDAG566 pDAG563 with G→A in central CG of internal parS* This work
pDAG567 pDAG565 with C→T in central CG of internal parS This work
pDAG568 pDAG564 with T→A mutation of promoter −10 (TAAAATa) in c3 parA This work
pDAG571 pDAG123 with single c1 parS at EcoRI upstream of pldc::lacZYA This work
pDAG572 pDAG123 with single c2 parS at EcoRI upstream of pldc::lacZYA This work
pDAG573 pDAG123 with single c3 parS at EcoRI upstream of pldc::lacZYA This work
pDAG574 pDAG123 with single p1 parS at EcoRI upstream of pldc::lacZYA This work
a

The mutated base is underlined.

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