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Spectra of NBD chloride-treated OhrR mutant proteins. Reduced C127S-C131S OhrR was treated with (solid line) or without (dotted line) an equivalent amount of CHP prior to incubation with 20 molar equivalents of NBD chloride. Shown are the UV-visible absorbance spectra of the NBD-labeled proteins with absorbance maxima at 347 and 420 nm, respectively.
