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DTNB analysis of free thiols in OhrR and mutant proteins under denaturing conditions
| OhrR (remaining cysteine[s]) | No. of free thiols per subunit
|
|
|---|---|---|
| Reduceda | Peroxide treatedb | |
| OhrR (C22, C127, C131) | 2.79 ± 0.22 | 0.82 ± 0.14 |
| C22S (C127, C131) | 1.89 ± 0.11 | 1.78 ± 0.16 |
| C127131S (C22) | 1.07 ± 0.04 | 0.010 ± 0.001 |
Proteins were prepared under reducing conditions.
Proteins were exposed to 1 equivalent of cumene hydroperoxide.
