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. 2006 Feb;188(4):1389–1395. doi: 10.1128/JB.188.4.1389-1395.2006

TABLE 2.

Masses of trypsin digest fragments of OhrR under oxidizing and reducing conditions

graphic file with name zjb00406549100t2.jpg
a

Z is free Cys modified with 4-vinylpyridine.

b

The location of the disulfide bond is deduced from the modification experiment with 2-bromoethylamine and NEM (see text).

c

C* is the S-2-aminoethylcysteine generated after treatment with 2-bromoethylamine; this residue is now a recognition site for trypsin that was cleaved on the C-terminal side of C*, proving the location of the disulfide bond between C22 and C127.