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Negative-ion MALDI-TOF MS spectra of lipid A isolated from Y. pestis KIM6+ (A), Y. pestis ΔmsbB (B), Y. pestis ΔmsbB (pLGmsbB) (C), Y. pestis ΔmsbB ΔlpxP (D), and Y. pestis ΔmsbB ΔlpxP(pLGlpxP) (E) grown at 21°C in LB. Peaks corresponding to lipid A species predicted to contain C10 (*), and aminoarabinose (†) are indicated (28). Acyl group positions are as proposed before (2, 23, 24).
