FIG. 8.

Analysis of the glycosylation of the Δ309-489 Ebola virus GP incorporated into pseudotyped retroviruses. Analysis was conducted as described in the legend to Fig. 4, except that aliquots of the samples were treated with PNGase F, with a combination of PNGase F, sialidase A, and endo-O-glycosidase, or with a combination of the previous three enzymes and β(1-4)-galactosidase and glucosaminidase. The migration positions of the mature GP₁ forms of the wild-type (WT) and Δ309-489 GPs are indicated. In this experiment, a glycosylated serum protein showing a mobility intermediate between those of wild-type GP₁ and Δ309-489 GP₁ was detected. The heterogeneous mobility of the PNGase F-treated proteins was indicative of the incomplete removal of N glycosylation. Neg, ΦNX cells transfected with pcDNA3 vector.