TABLE 1.
Effects of Xenopus MinK and MiRP2 on expression level dependence of Kv2.1 activation kinetics
| Sigmoidal (logistic) fit | A1 | A2 | x0 | slope | n | R2 |
| Kv2.1 cRNA | 181.9 ± 16.3 | 28.0 ± 5.1 | 0.77 ± 0.10 | 2.93 ± 0.80 | 223 | 0.53 |
| +control siRNA | 187.3 ± 9.4 | 56.8 ± 8.0 | 2.46 ± 0.28 | 3.41 ± 1.19 | 191 | 0.36 |
| +xMinK siRNA | 122.38 ± 7.24 | 38.8 ± 13.9 | 2.19 ± 0.44 | 4.23 ± 2.68 | 146 | 0.27 |
| Linear fit | A | B | R | SD | n | p |
| +xMiRP2 cRNA | 148.6 ± 10.6 | −36.4 ± 8.2 | −0.171 | 117.0 | 166 | 0.048 |
| +xMinK cRNA | 266.6 ± 14.7 | −70.5 ± 2.6 | −0.39 | 105.5 | 56 | 0.0029 |
| +xMiRP2 siRNA | 71.6 ± 4.2 | −37.8 ± 7.9 | −0.45 | 38.9 | 95 | <0.0001 |
| +xMiRP2/xMinK siRNA | 47.1 ± 1.8 | −15.2 ± 3.3 | −0.50 | 14.7 | 68 | <0.0001 |
Scatter plots were fit with a logistic dose response function, y = [A1–A2/1 + (x0/x)p] + A2, where x0 is the current at half-maximal effect (μA) and p is the slope factor; R2 is the coefficient of determination. Scatter plots that did not fit with a logistic dose response were fit with a straight line, y = Bx + A; values are also shown for correlation coefficient (R), standard deviation (SD), and significance level (p). In all cases, n = number of oocytes per group.