FIGURE 7.

Theoretical Markov simulation of unitary actomyosin interactions under varying dynamic loads. (a) 2.5 s of simulated events and (b) typical single simulated events. A time sequence of randomly occurring actomyosin events under varying dynamic load was generated with a Monte Carlo simulation using the model in the Appendix and was plotted as in Fig. 4. Similarly, forces on motor beads (dark colors) and the transducer beads (lighter colors) during actomyosin interactions simulated with the feedback gain of the isometric clamp at τ_f = 1 ms (blue), τ_f = 10 ms (red), and with the feedback turned off (green). The forces on beads for individual interactions were extracted from panel a and shown on an expanded timescale.