TABLE 3.

Temperature dependent enthalpy and corresponding heat capacity changes for protonation of the five paromomycin amino groups

Amino group	* (kcal/mol)	* (kcal/mol)	† (cal/mol K)
1	−15.8 ± 3.4	−7.9 ± 1.4	+392 ± 244
3	−9.0 ± 0.1	−8.2 ± 0.1	+38 ± 4
2′	−11.5 ± 5.0	−12.7 ± 3.6	−58 ± 430
2‴	−5.4 ± 1.7	−7.3 ± 2.2	−93 ± 199
6‴	−9.4 ± 0.1	−8.4 ± 0.1	+48 ± 12

^*Values of were derived from fits of the pH-dependent profiles shown in Fig. 8 using Eq. 4. The indicated uncertainties reflect the standard deviations of the experimental data from the fitted curves.

^†Values of were determined using Eq. 7 and the values listed in columns 2 and 3. The indicated uncertainties reflect the maximum errors in the values of as propagated through the Eq. 7.