TABLE 2.

Thermodynamic parameters for equilibrium unfolding of apoAI mutants

	Wild-type apoAI			del[1–43] apoAI			del[186–243] apoAI			[44–185] apoAI
Parameter	I	II	III	I	II	III	I	II	III	I	II	III
			2.4 ± 0.2			1.6 ± 0.1			3.5 ± 0.3			1.8 ± 0.1
			1.9 ± 0.2			1.0 ± 0.1			1.8 ± 0.1			2.0 ± 0.2
	4.5 ± 0.4	4.7 ± 0.4	4.3 ± 0.4	2.4 ± 0.2	2.2 ± 0.2	2.6 ± 0.2	5.1 ± 0.4	4.7 ± 0.4	5.3 ± 0.4	3.2 ± 0.3	3.4 ± 0.3	3.8 ± 0.3
mNI (kcal/mol)			2.1 ± 0.2			1.2 ± 0.1			3.1 ± 0.3			1.9 ± 0.2
mIU (kcal/mol)			1.4 ± 0.1			0.8 ± 0.1			2.1 ± 0.2			1.2 ± 0.1
mNU (kcal/mol)	3.6 ± 0.3	3.9 ± 0.3	3.5 ± 0.3	1.9 ± 0.2	1.8 ± 0.2	2.0 ± 0.2	5.1 ± 0.5	4.7 ± 0.5	5.2 ± 0.5	2.8 ± 0.2	2.6 ± 0.2	3.1 ± 0.3

I, II, and III refer to the three different methods used to calculate the change in free energy of the indicated transition; m is the GdnHCl susceptibility parameter and is given in Eqs. 11 and 12.