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Promoter escape leading edge FRET (Cy5, +20); 
distributions, calculated by averaging 
values for each single complex, taking into account only the time points where donor and acceptor probes are active. Transition from initiation (top) to elongation (bottom) results in the appearance of a new population at 
≈ 0.61. Inactive complexes are seen as an immobile population at 
≈ 0.3. Translocation activity is 72%.
