FIGURE 6.

Single-molecule time traces traces are plotted as a function of time, for different representative complexes (Cy5, +25). Only time points with active donors and acceptors are shown. The average values obtained for RP_itc,≤2 and RD_e,11 (0.11 and 0.5, respectively) are represented by two horizontal lines to guide the eye. (a) RP_itc,≤2, static distribution with ∼ 0.11 (see Fig. 5 a). (b) RP_itc,≤7, scattered distribution with a majority of time points distributed around a relatively high (t) value (distribution centered around = 0.3 for the active molecules (see Fig. 5 c)), consistent with the forward translocation of the leading edge of RNAP relative to downstream DNA during abortive initiation. (c) RD_e,11, static distribution with ∼ 0.5 (see Fig. 5 d).