Skip to main content
. 2005 Dec 9;90(5):1752–1766. doi: 10.1529/biophysj.105.071175

FIGURE 7.

FIGURE 7

Comparison of full-length KcsA SANS data at pH 7.5 and 5.0. (A) Increase in zero-angle scattering intensity of full-length KcsA at pH 5.0. (B and C) Guinier analysis and distance distribution at 0%, 22%, 41%, and 100% D2O solvent concentration. D(rmin) = D(rmax) = 0 was applied. (D) Protein-detergent match point at pH 5.0 (28% D2O). (E) Stuhrmann plot for full-length KcsA at pH 5.0. (F) Gel filtration analysis of full-length KcsA. Full-length KcsA was gel-filtered on a Shodex analytical gel filtration column at pH 7.5 and 5.0, respectively. Dashed line, pH 7.5; dotted line, pH 5.0 and buffer exchange on the gel filtration column; solid line, pH 5.0 and sample equilibration at pH 5.0 for 90 min before gel filtration. The void volume of this column is at 5.5 ml.