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Structural parameters of KcsA derived from SANS data at pH 7.5
| D2O | Full-length | Truncated | rmax (Å) | RG (Å) |
|---|---|---|---|---|
| 0% | x | 125 | 36.9 ± 0.4 | |
| 0% | x | 82 | 28.7 ± 0.3 | |
| 22% | x | 125 | 40.8 ± 1.0 | |
| 22% | x | 88 | 35.0 ± 0.5 | |
| 100% | x | 135 | 36.5 ± 0.3 | |
| 100% | x | 80 | 25.7 ± 0.2 |
The radii of gyration, RG, were calculated from the distance distribution using GNOM. rmax, maximum dimension of the macromolecule. The error in the determination of rmax with GNOM is generally of the order of 5%.
