TABLE 2.
Comparison of structural parameters of full-length KcsA at pH 7.5 and 5.0
| SAXS: full-length KcsA
|
SANS: full-length KcsA
|
|||||
|---|---|---|---|---|---|---|
| pH | RG (Å) | rmax (Å) | D2O | pH | RG (Å) | rmax (Å) |
| 7.5 | 41.9 ± 0.4 | 145.0 | 0% | 7.5 | 36.9 ± 0.4 | 125.0 |
| 5.0
|
68.1 ± 0.7
|
225.0
|
0% | 5.0 | 63.9 ± 0.6 | 200.0 |
| SAXS: truncated KcsA
|
22% | 7.5 | 40.8 ± 1.0 | 125.0 | ||
| pH | RG (Å) | rmax (Å) | 22% | 5.0 | 64.3 ± 1.2 | 200.0 |
| 7.5 | 34.5 ± 0.2 | 105.0 | 41% | 7.5 | 20.5 ± 0.1 | 68.0 |
| 5.0 | 34.6 ± 0.3 | 105.0 | 41% | 5.0 | 68.9 ± 0.9 | 209.0 |
| 100% | 7.5 | 36.5 ± 0.3 | 135.0 | |||
| 100% | 5.0 | 67.9 ± 0.7 | 205.0 | |||
All data were derived from distance distribution analysis using GNOM.