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. 2002 Nov;76(22):11226–11235. doi: 10.1128/JVI.76.22.11226-11235.2002

FIG. 7.

FIG. 7.

Overexpression of the N-terminal portion of Tsg101 inhibits virus particle release. (A) 293T cells were transfected with 5 μg of pSVRΔNB and either 50 μg of pTriEx1.1 (empty vector; lane 1), 25 μg of pTriEx5′Tsg plus 25 μg pTriEx1.1 (1:5 DNA ratio of pSVRΔNB to pTriEx5′Tsg; lane 2), or 50 μg of pTriEx5′Tsg (1:10 DNA ratio of pSVRΔNB to pTriEx5′Tsg; lane 3). At 48 h after transfection, virus particle samples were prepared as described in Materials and Methods and virus release was quantified by a reverse transcriptase assay. The data are the mean of three independent transfections. The error bars represent standard deviation. (B) Virus particle samples were also subjected to analysis by Western blotting using monoclonal antibody to HIV-2 p26. Lane numbering is as in panel A. The positions of marker proteins are indicated in kilodaltons.