FIG. 1.

Amino acid sequences of primate immunodeficiency virus Vpr proteins and mutants used in this study. (A) Alignment of the amino acid sequences of Vpr proteins from SIVcpz, HIV-1 NL4-3, SIVmac, SIVmne, HIV-2 Rod, SIVmnd, SIVagm, and SIVsyk. Sequences were obtained from the Los Alamos database. Gaps ( · ) were introduced to optimize the alignment. The conserved amphipathic α-helix and HxRxG motifs are highlighted with gray boxes. (B) Schematic representation of mutated amino acids in SIVmac239 Vpr. The consensus sequence is shown on top. Identical amino acids are indicated by dashes, and mutated amino acids are shown in capital letters.