FIG.3.

Subcellular localization of SIVmac239 Vpr and its mutant variants. Approximately 2 μg of pCMV6Myc expression plasmids for wild-type SIVmac239 Vpr or the derived α-helix, V21A, HxRxG1, HxRxG2, HxRxG1+2 P96T, or P96T mutant, as well as the empty vector, was introduced into COS cells. After 36 h, Myc epitope-tagged Vpr proteins were detected by using Cy3-conjugated anti-Myc antibodies and nuclei were stained with DAPI and visualized by fluorescence microscopy. Fluorescence pictures were processed, and anti-Myc staining was overlaid with DAPI (merge).