FIG. 7.

Effect of lysosomotropic agents on CD4 and CXCR4 surface levels. The effect of concanamycin A (dotted line) and BFLA-1 (dark solid line) on CD4 (top) and CXCR4 (bottom) expression was determined by flow cytometry with PE-labeled anti-CD4 or PE-labeled anti-CXCR4. Cells were incubated in the presence of the respective inhibitor for 3 h at 37°C, dislodged from the tissue culture plate with Versene, and stained at 4°C. For controls, cells treated with drugs were stained with an isotype-matched nonspecific immunoglobulin G1 PE-labeled antibody (thin dotted and dashed line), or cells were not treated with drugs, but stained with anti-CD4 or anti-CXCR4-PE labeled antibodies to determine the normal levels of surface expression (thin solid line).