TABLE 1.

Germ lines and V gene mutations in variable regions of anti-HCV E2 human monoclonal antibodies^a

Polypeptide chain	Antibody	V gene	% of mutated nucleotides in:		% of mutated amino acids in:
			FRs	CDRs	FRs	CDRs
Heavy	e8	VH1-18	9.5	22.2	14.9	33.3
	e20	VH1-69	9.4	16.9	19	38
	e137	VH1-69	11.5	15.3	14	41.7
	e301	VH1-69	8.9	19.4	15.6	45.8
	e509	VH1-69	5.2	15.9	10.9	33.3
Light	e8	KV3-20	2.7	16	2.6	33.3
	e20	KV1-9	4.3	7.7	9.7	22.2
	e137	KV1-8	2.2	9	3.2	15.4
	e301	KV3-15	3.8	14.3	9.7	23
	e509	KV3-15	3.2	1.3	6.5	0

^aSequences were determined as described previously (7) and aligned with germ line sequences in the IMGT database (11). The percentages of nucleotide and amino acid mutations were calculated according to the alignment method of Kabat et al. (9), taking into account framework regions (FRs) 1, 2, and 3 for heavy and light chains and complementarity-determining regions (CDRs) 1 and 2 for heavy chains and 1, 2, and 3 for light chains.