FIG. 2.

(A) Western blot analysis of nuclear extracts from sf1Ep cells transfected with CRPV wt E2-pSG5 or one of the E2 mutants R37K-, R37A-, E39Q-, E39A-, I73L-, and I73A-pSG5. The control lane contains an extract from cells transfected with the parental pSG5 vector. The position of the E2 proteins is indicated by an arrow. A molecular-size marker (in kilodaltons) is shown on the left. (B) Electromobility shift assay of CRPV wt and mutant E2 proteins present in nuclear extracts of transfected sf1Ep cells. Control lanes contained no protein extract (oligo) or an extract from cells transfected with the parental vector (pSG5). The retarded bands corresponding to the E2-DNA complex (a and b) and the unbound ³²P-labeled oligonucleotide containing an E2 binding site (f) are indicated on the right.