TABLE 1.
Primers used in the RFLP analysis in this studya
| Name | Sequence | Position (nucleotide range) |
|---|---|---|
| 01-N12 | 5′-ATTGTATGCATGCGATTGCTATCGC-3′ | 5372-5396 |
| 01-R13 | 5′-GGTCTTCCACTTTAAAGGGGTTTGC-3′ | 6134-6110 |
| 50-N15 | 5′-CGATCACGTCGCTCACATCCAACCC-3′ | 93685-93709 |
| 50-R17 | 5′-ATGGCAGAAGAAACACGTATTGCCG-3′ | 94457-94433 |
| 60-N06 | 5′-GAGGACAACAGCTCCACCTTGACCG-3′ | 105277-105301 |
| 60-R06 | 5′-GAGTAATGTGGCCGCCCGGTTTTGG-3′ | 105613-105589 |
| 60-N26 | 5′-CCAAAACCGGGCGGCCACATTACTC-3′ | 105589-105613 |
| 60-R28 | 5′-ATTACTGTCGACCCGAGACCTGGCC-3′ | 106380-106356 |
| 60-N11 | 5′-AAGGGCTTCCGTCGGGCATCATGAG-3′ | 107729-107753 |
| 60-R12 | 5′-TCGGGTAAAAAGCCGGGCGATGAGC-3′ | 108497-108473 |
a
These primers were designed by referring to the nucleotide sequence of the Dumas strain. Nucleotide positions refer to those in the sequence of the Dumas strain.