FIG. 4.

Anti-HIV activity of SAMMA in U87 CD4⁺ CCR5⁺ cells infected with the R5 virus virus HIV-BaL (A) and in U87 CD4⁺ CXCR4⁺ cells infected with the X4 isolate IIIB (B). U87 cells were preincubated with the indicated SAMMA concentrations or control medium for 1 h at 37°C and then subjected to a 2-h infection with virus. After 2 h, the cells were washed twice with PBS to remove unbound virus and compound. Viral replication was monitored by measuring p24 protein in culture supernatants by ELISA 48 h postinfection. Results are the mean p24 values from triplicate wells and are representative of at least two independent experiments; error bars indicate standard deviation.