TABLE 5.
Intracellular cytokine staining in peripheral blood and mucosal (distal-colon) tetramer+ CD8+ lymphocytes from DNA-primed and recombinant-adenovirus-boosted rhesus monkeys following in vitro stimulation
| Monkey | % p11C tetramer+ IFN-γ+ CD8+ T cells
|
|||
|---|---|---|---|---|
| Peripheral blood lymphocytes
|
Distal-colonic lymphocytes
|
|||
| Unstimulateda | PMA + Ionob | Unstimulated | PMA + Iono | |
| AW28 | 0.40 | 98.91 | 3.40 | 97.93 |
| AW2P | 0.00 | 98.37 | 3.00 | 94.28 |
a
Peripheral blood and colonic lymphocytes containing brefeldin A (10μg/ml) were cultured for 6 h at 37°C suspended in RPMI containing 12% fetal calf serum.
b
Iono, ionomycin.