Abstract
The DNA sequences which code for ribosomal DNA have been isolated and purified. The technique used has general application for RNA:DNA hybridization studies and enables the isolation of any gene for which sufficient gene product can be obtained. Experiments with isolated ribosomal RNA cistrons demonstrated that (a) the majority of the ribosomal cistrons are similar to one another; (b) the cistrons which are similar to one another are virtually identical to one another; (c) ribosomal cistrons of different bacterial species are closely related to one another.
Full text
PDF














Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ARONSON A. I., HOLOWCZYK M. A. COMPOSITION OF BACTERIAL RIBOSOMAL RNA. HETEROGENEITY WITHIN A GIVEN ORGANISM. Biochim Biophys Acta. 1965 Feb 8;95:217–231. doi: 10.1016/0005-2787(65)90487-9. [DOI] [PubMed] [Google Scholar]
- BOLTON E. T., MCCARTHY B. J. FRACTIONATION OF COMPLEMENTARY RNA. J Mol Biol. 1964 Feb;8:201–209. doi: 10.1016/s0022-2836(64)80129-7. [DOI] [PubMed] [Google Scholar]
- BOLTON E. T., McCARTHY B. J. A general method for the isolation of RNA complementary to DNA. Proc Natl Acad Sci U S A. 1962 Aug;48:1390–1397. doi: 10.1073/pnas.48.8.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bernardi G. Chromatography of nucleic acids on hydroxyapatite. Nature. 1965 May 22;206(4986):779–783. doi: 10.1038/206779a0. [DOI] [PubMed] [Google Scholar]
- Brenner D. J., Cowie D. B. Thermal stability of Escherichia coli-Salmonella typhimurium deoxyribocleic acid duplexes. J Bacteriol. 1968 Jun;95(6):2258–2262. doi: 10.1128/jb.95.6.2258-2262.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
- CHAMBERLIN M., BERG P. MECHANISM OF RNA POLYMERASE ACTION: FORMATION OF DNA-RNA HYBRIDS WITH SINGLE-STRANDED TEMPLATES. J Mol Biol. 1964 Feb;8:297–313. doi: 10.1016/s0022-2836(64)80139-x. [DOI] [PubMed] [Google Scholar]
- Clausen T. Measurement of 32P activity in a liquid scintillation counter without the use of scintillator. Anal Biochem. 1968 Jan;22(1):70–73. doi: 10.1016/0003-2697(68)90260-1. [DOI] [PubMed] [Google Scholar]
- DOI R. H., IGARASHI R. T. CONSERVATION OF RIBOSOMAL AND MESSENGER RIBONUCLEIC ACID CISTRONS IN BACILLUS SPECIES. J Bacteriol. 1965 Aug;90:384–390. doi: 10.1128/jb.90.2.384-390.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Doi R. H., Igarashi R. T. Heterogeneity of the conserved ribosomal ribonucleic acid sequences of Bacillus subtilis. J Bacteriol. 1966 Jul;92(1):88–96. doi: 10.1128/jb.92.1.88-96.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dubnau D., Smith I., Morell P., Marmur J. Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid base sequence homologies. Proc Natl Acad Sci U S A. 1965 Aug;54(2):491–498. doi: 10.1073/pnas.54.2.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KOTAKA T., BALDWIN R. L. EFFECTS OF NITROUS ACID ON THE DAT COPOLYMER AS A TEMPLATE FOR DNA POLYMERASE. J Mol Biol. 1964 Aug;9:323–339. doi: 10.1016/s0022-2836(64)80210-2. [DOI] [PubMed] [Google Scholar]
- MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
- MIYAZAWA Y., THOMAS C. A., Jr NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES. J Mol Biol. 1965 Feb;11:223–237. doi: 10.1016/s0022-2836(65)80053-5. [DOI] [PubMed] [Google Scholar]
- Moore R. L., McCarthy B. J. Comparative study of ribosomal ribonucleic acid cistrons in enterobacteria and myxobacteria. J Bacteriol. 1967 Oct;94(4):1066–1074. doi: 10.1128/jb.94.4.1066-1074.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OTAKA E., MITSUI H., OSAWA S. On the ribonucleic acid synthesized in a cell-free system of Escherichia coli. Proc Natl Acad Sci U S A. 1962 Mar 15;48:425–430. doi: 10.1073/pnas.48.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Walker P. M., McLaren A. Fractionation of mouse deoxyribonucleic acid on hydroxyapatite. Nature. 1965 Dec 18;208(5016):1175–1179. doi: 10.1038/2081175a0. [DOI] [PubMed] [Google Scholar]
