TABLE 1.
Segment | Primer sequence | Positiona | Orientation |
---|---|---|---|
5′LTR | 5′-CTACCACACACAAGGCTACT-3′ | 53-72 | 5′ |
5′LTR | 5′-CTCGCACCCATCTCTCTCCTT-3′ | 779-799 | 3′ |
1 | 5′-AGTCAGTGTGGAAAATCTCT-3′ | 611-632 | 5′ |
1 | 5′-GCTGAAAGCCTTCTCTTCTACTA-3′ | 1261-1283 | 3′ |
2 | 5′-TAGTAGAAGAGAAGGCTTTCAGC-3′ | 1261-1283 | 5′ |
2 | 5′-GTCCTTCCTTTCCACATTTCC-3′ | 2028-2049 | 3′ |
3 | 5′-TGGAAATGTGGAAAGGAAGGAC-3′ | 2028-2049 | 5′ |
3 | 5′-CCTAATTGAACTTCCCAGAA-3′ | 2807-2826 | 3′ |
4 | 5′-TTCTGGGAAGTTCAATTAGG-3′ | 2807-2826 | 5′ |
4 | 5′-ACTGCCTCTGTTAATTGTTT-3′ | 3644-3663 | 3′ |
5 | 5′-GCAAGGCCAATGGACATATCAA-3′ | 3547-3568 | 5′ |
5 | 5′-CTACAGTCTACTTGTCCATGCA-3′ | 4377-4398 | 3′ |
6 | 5′-GATGGAATAGATAAGGCCCAAGA-3′ | 4271-4290 | 5′ |
6 | 5′-CATCCTGTCTACTTGCCACACA-3′ | 5047-5066 | 3′ |
7 | 5′-GGAAAGGACCAGCAAAGCT-3′ | 4932-4940 | 5′ |
7 | 5′-CTGCTATGTCGACACCCAAT-3′ | 5778-5797 | 3′ |
8 | 5′-ATTGGGTGTCGACATAGCAG-3′ | 5778-5797 | 5′ |
8 | 5′-GATGCACAAAATAGAGTGGTGGT-3′ | 6368-6390 | 3′ |
9 | 5′-GATATGAGGGACAATTGGAGAA-3′ | 7643-7664 | 5′ |
9 | 5′-CTGTCTCTGTCTCTCTCTCCA-3′ | 8434-8454 | 3′ |
10 | 5′-GTGAATAGAGTTAGGCAGGGAT-3′ | 8336-8357 | 5′ |
10 | 5′-AGTAGCCTTGTGTGTGGTAG-3′ | 9137-9156 | 3′ |
3′LTR | 5′-GCTAGAAGCACAAGAGGAGGA-3′ | 8966-8986 | 5′ |
3′LTR | 5′-CTAGAGATTTTCCACACTGACT-3′ | 9695-9716 | 3′ |
Primer position according to the HXB2 provirus sequence numbering.