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. 2026 Oct 8;7(4):104891. doi: 10.1016/j.xpro.2026.104891

Figure 4.

Figure 4

Characterization of evRSPO1

(A) Size distribution of evRSPO1.

(B) TEM images of evRSPO1.

(C) Western blot analysis of RSPO1, His tag, HSP70, Calnexin, and CD63 in total cell lysate, culture supernatant (Media), large EVs, and sEVs from the RSPO1-overexpressing HEK293 cell line.

(D) Immunogold labeling analysis of evRSPO1, with red arrows indicating the RSPO1 protein.

(E) Single-molecule imaging of RSPO1 and CD63 in evRSPO1 using the dSTORM system.