Figure 6.

evRSPO1 treatment promotes intestinal tissue regeneration after injury and aging

(A) Representative confocal fluorescence images of PKH26-labeled EV and FITC-labeled phalloidin in the mouse intestine after evRSPO1 treatment.

(B) Percent survival curve of radiation-induced intestinal injury in mice after evWT or evRSPO1 treatment (n = 8).

(C) Representative H&E staining of intestinal tissue from radiation-induced injury mice after evWT or evRSPO1 treatment.

(D) Quantification of the length of crypts from (C) (n = 8).

(E) Representative β-galactosidase staining of intestinal tissue from young or old mice after evWT or evRSPO1 treatment.

(F) Quantification of the positive area of β-galactosidase from (E) (n = 8).

(G) Principal component analysis (PCA) of gene expression profiles, showing sample separation by group.

(H) Heatmap showing cell cycle-related gene expression patterns in the intestinal tissue from young mice and old mice after evWT or evRSPO1 oral administration for 2 weeks. Statistical data are presented as mean ± SD. Two-tailed unpaired Student’s t-tests were used to compare the two groups. The p-values were calculated and evaluated for statistical significance (∗∗p < 0.01 and ∗∗∗∗p < 0.0001).