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Intestinal organoid medium
| Reagent | Final concentration |
|---|---|
| Advanced DMEM/F12 | N/A |
| RSPO1 recombinant protein | 500 ng/mL |
| EGF recombinant protein | 50 ng/mL |
| Noggin recombinant protein | 100 ng/mL |
| N2 | 1× |
| B27 | 1× |
| GlutaMAX | 1× |
| N-acetylcysteine | 1 mM |
| penicillin/streptomycin | 1% |
