FIG. 3.

Nipah virus V protein prevents nuclear import of STAT1 and STAT2. (A) 2FTGH cells were transfected with empty vector or HA-tagged Nipah V expression plasmid (+NV) and were either unstimulated (UNT) or treated for 30 min with 1,000 U of IFN-α per ml (+IFNα). Cells were fixed, permeabilized, and stained sequentially for HA and then for STAT1 or STAT2. (B) Independent block on STAT1 translocation. Same as in panel A, but the cells were treated for 30 min with 5 ng of IFN-γ per ml prior to processing for NV (HA), STAT1, and STAT2 fluorescence. Left panels indicate the parental cell line 2FTGH (WT). Right panels indicate the STAT2-deficient daughter cell line, U6A (STAT2⁻). Arrows indicate Nipah V protein-expressing cells.