FIG. 5.

Nipah virus V protein inhibits activating tyrosine phosphorylation of STAT1. Individual 60-mm plates of 293T cells were transfected with 15 μg of empty vector or FLAG-tagged Nipah virus V expression vector (NV). Cells were treated 24 h later with 1,000 U of IFN-α per ml or 5 ng of IFN-γ per ml for 30 min prior to lysis. Lysates (50 μg of total protein) were separated by SDS-PAGE (7% polyacrylamide), transferred to nitrocellulose, and visualized by immunoblotting with antisera to STAT1, phosphotyrosine 701-modified STAT1 (STAT1-P), and FLAG epitope tag.