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. 2002 Nov;76(22):11518–11529. doi: 10.1128/JVI.76.22.11518-11529.2002

FIG. 1.

FIG. 1.

Expression of the E protein by a Sindbis virus replicon vector in BHK-pAPN cell lines. (A) Scheme of the Sindbis virus replicon construct encoding the TGEV E gene. RSV Pr, Rous sarcoma virus promoter; sgRNA, subgenomic RNA; Pac, puromycin resistance gene; SV40 poly A, transcription-termination polyadenylation signal from simian virus 40. nsP1-4, nonstructural proteins 1 to 4; nsP2mut, mutant nonstructural protein 2. (B) Western blot analysis of E protein expression at different cell passages (P) of BHK-pAPN transformed with the Sindbis virus replicon expressing the E protein. The position of the E protein is indicated by an arrow. Mock, untransfected. (C) Immunofluorescence microscopy using an E protein-specific MAb at different cell passages of BHK-pAPN transformed with the Sindbis virus replicon expressing the E protein.