FIG. 2.

TGEV growth kinetics and rescue from cDNA in BHK-pAPN cells expressing E protein using Sindbis replicon. (A) Growth kinetics of TGEV in BHK-pAPN cells expressing TGEV E protein. Virus titers were determined by plaque assay. (B) Titration of infectious TGEV recovered from a transfected cDNA in BHK-pAPN cells expressing TGEV E protein by Sindbis virus replicon. E, BHK-pAPN cells expressing the TGEV E protein. SIN, cells transfected with pSINrep21 plasmid. C, mock-transfected cells. Mean values from four experiments are represented, and standard deviations are shown as error bars.