FIG. 1.

HCMV US10 associates with MHC class I HC. (A) US10-HA-expressing cells and control cells were metabolically labeled for 50 min. Folded MHC class I-β₂m complexes, US10-HA, and free HC were recovered from cell lysates by immunoprecipitation (IP) with W6/32, 12CA5, and anti-HC antibodies (αHC), respectively. A reimmunoprecipitation(re-IP) for HC was performed from a portion of the 12CA5 immunoprecipitates that had been denatured in 1% SDS and boiled (lanes 3 and 7). The positions of prestained molecular size standards (Amersham) are shown on the right. (B) US10-HA and control cells were metabolically labeled for 10 min and chased for up to 45 min in nonradioactive medium. HC and US10-HA molecules were recovered from cell lysates with anti-HC and 12CA5 antibodies, respectively. All samples were analyzed by SDS-PAGE (12.5% polyacrylamide gels).