FIG. 7.

Effect of replacing S186 with a negatively charged amino acid on the ability of ZEBRA to bind DNA. Ten micrograms of total cell protein from HKB5/Cl8 cells transfected with the indicated plasmids were tested for their ability to bind a radiolabeled oligonucleotide containing a ZIIIB site by an electrophoretic mobility shift assay. The ZEBRA-DNA complex was identified by supershift (SS) analysis using a polyclonal antibody against ZEBRA (A) or the BZ1 monoclonal antibody (B). +, present; −, absent.