FIG. 6.

RT-nested-PCR analysis of HPV31b spliced E8∧E2C transcripts expressed following infection of HaCaT cells. Details of the experiment are given in the legend to Fig. 3. (A) First round of RT-PCR using primers E1.4A and E4B to illustrate the two amplicons present in CIN-612 9E cells. (B) Nested PCR using primers E1A and E4.4B. For each reaction, the input RNA in the first round of PCR corresponded to 1.9 μg. (C) Early region of the HPV31b genome and explanation of observed RNA structures as described in the legends to Fig. 1 and 3C. Boldface letters denote previously characterized ORFs, with their splice junctions indicated in parentheses. The novel RNA structure containing the 1124∧3295 splice junction is shown. The E8∧E2C structure was verified by sequencing from two positive samples in panel B, and the novel 1124∧3295 splice junction was characterized by sequencing of products from 9E cells.