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. 2002 Nov;76(22):11343–11349. doi: 10.1128/JVI.76.22.11343-11349.2002

FIG. 2.

FIG. 2.

Southern blot analyses of rAAV vector genomes in the liver. Vector forms in AAV-hF.IX16-injected mouse liver harvested 14 weeks postinjection (A) and AAV-EF1α-nlsLacZ-injected mouse liver harvested 28 weeks postinjection (B) are shown. Twenty micrograms of total DNA was digested with SacI or NsiI (which does not cut the vector genome), separated on a 0.8% agarose gel, blotted, and probed with a vector sequence-specific probe. Lane 1.0 represents a 1.0-vg/dge standard (a 7.0-kb plasmid containing AAV-hF.IX16 vector sequence and a SacI recognition site for panel A and a 6.7-kb plasmid containing AAV-EF1α-nlsLacZ vector sequence and an NsiI recognition site for panel B), showing complete digestion of the DNA with the restriction enzyme used. Each lane represents an individual mouse. Open and filled arrowheads indicate supercoiled ds circular rAAV vector genomes and concatemers, respectively. (A) Lane 1, 1.0 × 1011; lane 2, 3.0 × 1011; lane 3, 1.8 × 1012; and lane 4, 1.1 × 1013 vg/mouse. (B) Lane 1, 5.0 × 1010; lane 2, 3.0 × 1011; lane 3, 1.8 × 1012; and lane 4, 3.9 × 1012 vg/mouse.