Alignment of the 21 PERV γ1 pro-pol clones amplified in this study. The amplified clones assigned to γ1A (A) and γ1B (B) and the published GenBank sequences which showed the highest identity are shown. The polymorphic nucleotides (n = 16 [1.7%] and n = 44 [4.7%] for γ1A and γ1B, respectively) of the amplified 928-nt pro-pol sequence are depicted, with the numbers of their positions in the alignment of the respective data set shown at the top. Mutant nucleotides are depicted in shaded boxes. Fragments harboring an ORF are indicated with an asterisk. Sequence A66552 represents seven GenBank sequences which are highly homologous in the pro-pol region and are identical at nt A423 (A66553, AF038601, AF147808, AJ133817, AJ279057, and U77599). (C) Sequence comparison of the clones m317, m1120, m3111, and m3112 to other PERV γ1 sequences amplified in this study or taken from GenBank. The three hybrid clones m1120, m3111, and m3112 are depicted in bold letters. Homologous regions are shown in boxes, and polymorphic nucleotides of the hybrid sequences are shown in shaded boxes.