FIG. 1.

Vaccinated animal 87082 makes CD8-positive T-cell responses to regions of viral Gag and Nef proteins. At 1 week after the boost with recombinant MVA expressing SIV proteins, PBMC from animal 87082 were isolated and tested in ICS against pools of 15mer peptides (A and C), as described in Materials and Methods. (B and D) If a peptide pool sensitized cells to produce IFN-γ and CD69 (see Gag pool B and Nef pool D), its constituent peptides were then tested with PBMC from the second week after immunization. The 15mer peptides Gag 17 and 18 (B) and Nef 63 and 64 (D) were found to stimulate CD8-positive lymphocytes. The sequences of the regions where these peptides overlap are boxed in dark gray.