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. 2002 Nov;76(22):11623–11636. doi: 10.1128/JVI.76.22.11623-11636.2002

FIG. 2.

FIG. 2.

Definition of minimal optimal CD8-positive T-cell epitopes. T-cell lines specific for Gag 17 and Nef 64 were generated and tested in ICS against a range of concentrations of overlapping peptides in order to determine which peptide results in maximal stimulation of the T-cell line. The two 9mer peptides, Gag 17-4 (GSENLKSLY; Gag71-79 GY9) and Nef 139 (YTSGPGIRY; Nef159-167 YY9), optimally stimulated the Gag 17-specific (A) or the Nef 64-specific T cells (B).