FIG. 4.

Pooled motif and individual ligand sequences for Mamu-A*02. (A) Peptides eluted from Mamu-A*02 were pooled and subjected to Edman sequencing. The resultant data were analyzed as previously described (6) to generate a peptide motif based on the fold increase in picomoles over the prior round of sequencing. Dominant residues (in boldface) exhibited a ≥3.5-fold increase over the prior round; strong residues (in blue) exhibited a 2.5- to 3.5-fold increase; weak residues (in green) exhibited a 2.0- to 2.5-fold increase. (B) Individual ligand sequences were derived from MS/MS sequencing of selected ions. The two new Gag and Nef SIV epitopes and the previously described Env epitope (39) are listed below the naturally loaded ligands.