FIG. 7.
Mutant peptides reduce recognition by Nef159-167 YY9-specific CTL. T-cell lines specific for Nef159-167 YY9 were generated from four Mamu-A*02-positive, SIV-infected animals by using thawed PBMC from weeks 4 (animal 87082), 6 (animal 95084), 8 (animal 96020), or 10 (animal 97086) postchallenge. These T-cell lines were then tested in ICS against a range of concentrations of the index peptide and variant peptides representing some of the mutant epitope sequences detected in these animals (see Fig. 6).