FIG. 2.

Migration of PBL and HOSCD4X4R5 cells in response to chemokines. Different concentrations of SDF-1α (top panels) or MIP-1β (bottom panels) were placed in the lower wells of the chemotaxis chamber; cells were placed in the upper wells, which were separated from the lower wells by a polycarbonate filter. The results are expressed as chemotaxis index representing the fold increase of migrating cells in response to chemokines over the response to control medium. Significant cell migration (P < 0.05) was detected with 10 ng of chemoattractant/ml. Gray bars, untreated cells; black bars, cells treated with 10 mM MβCD for 30 min at 37°C; white bars, MβCD-treated cells loaded with 75 μg of cholesterol/ml complexed with MβCD. (A) PBL; (B) HOSCD4X4R5.