FIG. 4.

Inhibition of HNS2 neutralization of virus pseudotyped with R2 envelope by synthetic cyclized R2 V3 35-mer peptide. (A) The results shown indicate the average luminescence units obtained from triplicate determinations. The data points correspond to peptide concentrations, as indicated in the box insert. The lines shown are best-fit trend lines for each peptide concentration, which were calculated using Microsoft Excel. (B) The results shown indicate the means and SDs of luminescence units obtained in the absence (▪) and presence of peptide, 0.06 (□) and 0.5 (○) μg/ml, respectively. (C) The results shown were obtained when the trend lines shown in panel A were used to calculate 90% serum neutralizing concentrations corresponding to each concentration of peptide. The results are presented to show the relationship between peptide concentration and residual serum neutralizing activity. The best-fit trend line shown (heavy line) and the regression coefficient obtained (R²) were calculated in Microsoft Excel. The ID₅₀ and fold inhibition at maximum peptide concentration were determined using the line equation for the best-fit line.