TABLE 1.
Energy transfer efficiency values for donor-acceptor pairsa
| Donor (Cy3) | Acceptor (Cy5)/cell line | Mean E ± SD (%) |
|---|---|---|
| MHC-I | β2-microglobulin/GMK | 38 ± 1.5 |
| Transferrin receptor | GRP78/GMK | 1.0 ± 1.5 |
| GRP78 | CAV-9/GMK | 25 ± 0.5 |
| CAV-9 | GRP78/GMK | 23 ± 1.0 |
| MHC-I | GRP78/GMK | 21 ± 0.8 |
| MHC-I | GRP78 (in the presence of CAV-9)/GMK | 31 ± 0.5 |
| MHC-I | GRP78/Daudi | 0.5 ± 0.5 |
| MHC-I | GRP78/Daudi-MHC+ | 25 ± 1.5 |
a
Energy transfer between different pairs was detected from the increase in donor fluorescence after acceptor photobleaching. Data are from a number of independent experiments.