FIG. 1.
Time course of P131 and P191 synthesis in BY-2 tobacco protoplasts. Protein extracts of mock-infected (lanes 1 and 7) or PCV-infected protoplasts (lanes 2 to 5 and lanes 8 to 12) harvested at the time (hours) postinfection indicated at the bottom were analyzed by Western blotting. The proteins were separated by sodium dodecyl sulfate-8% polyacrylamide gel electrophoresis, transferred onto an Immobilon-P membrane, and probed with antisera raised against P131 (lanes 1 to 5) or P191 (lanes 7 to 10) or with affinity chromatography-purified anti-P131 (lane 11) or anti-P191 (lane 12) antibodies. Bound antibodies were visualized by chemiluminescence. Lane 6 correponds to in vitro [35S]methionine-labeled translation products obtained after incubation of viral PCV RNA in a wheat germ extract. The positions of P131 and P191 are indicated by arrows.